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OncoSpan - The World's Largest Reference Standard

OncoSpan is Horizon’s largest Reference Standard, which features 386 variants across 152 key cancer genes. All our Reference Standards are cell line-derived for the closest representation of your patient sample. You also have access to batch-specific NGS data for all variants, providing you with a high-quality truth set to give you consistency, confidence and control over your assays.

OncoSpan Product Details

Features:

  • World's largest oncology Reference Standard
  • Cell line-derived for the closest representation of your patient sample
  • High coverage across the top NGS gene panels, cross-checked against amplicon design
  • Batch-specific VCF file included with every purchase
  • 25 variants are orthogonally validated by ddPCR, with a range of allelic frequencies
  • 195 variants with a COSMIC ID

Genes and Variants

  • 152 Genes
  • 386 Variants
  • INDELs: 24 deletions, 6 insertions (ranging from 2 to 16 base pairs)

OncoSpan covers 152 genes

ABL1
AKT1
AKT2
ALK
APC
AR
ARID1A
ATR
ATRX
AXL
BARD1
BCL6
BLM
BMPR1A
BRAF
BRCA1
BRCA2
BTK
BUB1B
CARD11
CCND1
CCND3
CCNE1
CD79B
CDH1
CDK12
CDK4
CEP57
CFH
CREBBP
CSF1R
CTNNB1
DDR2
DIS3L2
DNMT3A
EGFR
EML4
EP300
EPCAM
ERBB2
ERBB3
ERCC1
ERCC2
ERCC4
ERCC5
ERG
ETS1
ETV4
EWSR1
EXT1
FANCA
FANCD2
FANCE
FANCG
FANCI
FANCM
FBXW7
FGF10
FGF2
FGF3
FGF6
FGFR1
FGFR3
FLCN
FLI1
FLT1
FLT3
FZR1
GATA2
GATA3
GEN1
GNA11
GNAS
HNF1A
HRAS
IDH1
IDH2
JAK1
JAK2
JAK3
KDR
KIT
KRAS
LDLR
MAGI1
MAP2K1
MAP2K2
MAX
MDM4
MED12
MET
MLH1
MLLT3
MMAB
MRE11
MSH2
MSH3
MSH6
MTOR
NBN
NF1
NFE2L2
NOTCH1
NOTCH2
NOTCH3
NRAS
NRG1
NTRK1
NTRK3
PDGFRA
PDGFRB
PIK3CA
PIK3CD
PIK3CG
PIK3R1
PMS2
PPARG
PPP2R2A
PRKAR1A
PROC
PTCH1
PTPN11
RAD51B
RAD54L
RAF1
RB1
RBM45
RECQL4
RET
RHBDF2
ROS1
RPS6KB1
SDHB
SF3B1
SF3B2
SLTM
SLX4
SMARCB1
SMO
SMOX
STK11
TERT
TET2
TFRC
TP53
TP53BP1
TSC1
TSC2
WRN
XPA
XPC
ZNF395

 

 

OncoSpan is designed to work alongside the top NGS gene panels, to streamline oncology genetics. All our variants have been cross-checked against the amplicon design in these popular gene panels from Thermo Fisher Scientific and Illumina.

Thermo Fisher Scientific

  Genes in panel Genes covered by OncoSpan Coverage in %
Cancer Hotspot Panel (v2) 50 12 24%
Oncomine Colon cfDNA 14 7 50%

 

Illumina

  Genes in panel Genes covered by OncoSpan Coverage in %
Oncomine Lung cfDNA 11 6 55%
Trusight Tumor 15 15 9 60%
Trusight Tumor 26 26 13 50%
Trusight Tumor 170 170 74 44%
Trusight Cancer 94 36 38%

 

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Product Characterization

High coverage (500x) exome sequencing is performed on every batch of OncoSpan, providing accurate characterization of the background genome.

Agilent SureSelectXT Human All Exon V6 with Illumina sequencing is performed under strict quality controls (ISO 9001, ISO 17025) to provide you with an accurate and reliable NGS data set for your product.

Additionally, 25 variants are orthogonally validated by ddPCR, with a range of allelic frequencies. 

 

Product Specification

Catalog Number HD827
Format gDNA
Amount of DNA 1μg
Volume 20μl
Concentration 50ng/μl
Quality certificates ISO 9001, ISO 13485

 

Buy OncoSpan Now!

All of our OncoSpan information is available in a downloadable PDF.