Detecting the Onset and Kinetics of Apoptosis

Download the application note to learn how different apoptosis assays can be used to analyze cell killing capacity of your compound of interest.

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In this application note we:

Used three different readouts to analyze the kinetics of apoptosis in response to staurosporine and etoposide in cancer cell lines:

  • IncuCyte live cell imaging to track the appearance of a fluorescent caspase-3/7 dye
  • Multiplexing a cell viability dye with a caspase-3/7 dye in a plate reader-based assay
  • Analysing early and late apoptotic cell markers using western blotting and flow cytometry